Transcriptional Enhancers - Looking out for the Genes and Each Other

on YouTube ↗35m

" summary

A coupled hierarchical Bayesian model integrates epigenomic enhancer priors and enhancer–gene linking features to fine-map eQTLs in ~300 human hearts, outperforming lasso and fgwas-like approaches in explained variance and recovering variants with DNase footprints and motif disruption enrichment. Genome-wide DNase correlation finds distal enhancer pairs that share TF motifs, chromatin-modifier links and Hi-C proximity, and a facilitated-diffusion extension predicts an occupancy boost ~200% for spatially clustered homotypic sites, validated against matched chromatin and GC controls.

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" chapters (12)

1. 0:00Intro
2. 0:21Transcriptome - a key mediator of diversity
3. 1:09Transcriptional Regulation
4. 1:51Enhancers - key mediators of context-specific gene regulation
5. 3:15Genome-wide association studies
6. 3:43Expression Quantitative Trait Loci (eQTL)
7. 11:48Biology: Protein binding
8. 14:46Allelic imbalance
9. 17:15Properties of correlated enhancers
10. 22:40Determinants of TF-DNA interaction
11. 24:32Computing occupancy 'boost in archipelagos (APs) with digital footprint data
12. 28:23Downstream impact of crowdsourcing occupancy boost

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