Cross-Species Interactome Mapping Reveals Network Evolution Principles from Yeasts to Human

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Built a comprehensive fission yeast binary interactome by screening 75 million pairs (5,000 × 5,000 × 3) and reporting about 2,300 interactions across 1,300 proteins, roughly half novel and ~700 linking to human orthologs. Primary yeast two‑hybrid screens are retested and validated with a protein complementation assay in mammalian cells; measured false negatives explain sparse overlaps in literature maps. Corrected conservation estimates show ~40% Pombe–budding conservation and ~70% Pombe–human, while nearly half conserved links are functionally rewired at the interface.

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" chapters (13)

1. 0:00Intro
2. 0:27Evolution of protein functions
3. 2:10Evolution of protein interactions
4. 9:28Systematic well-controlled cross- species interactome mapping
5. 11:00Interactome quality
6. 12:58Heat shock gene regulation by Atf1
7. 20:34Outline
8. 21:15Conservation rate of interactions across species
9. 26:52Many functions shared between fission yeast and human are lost in budding yeast
10. 27:04Conservation paradox
11. 27:57Cross-species interactome mapping
12. 29:15Half of the conserved interactions have actually co-evolved to potentially accommodate new partners and functions.
13. 31:08Whole genome duplication vs. Small-scale gene duplication