Variation in Regulatory Information Within and Between Species

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" summary

Maps chromatin marks in 19 people and finds enhancer marks most variable while promoters are more stable; activity frequently toggles on/off but enhancers rarely convert to promoters, and many variable sites link to motif-disrupting SNPs and show partial heritability. Measures ~6,000 proteins across ~90 lymphoblastoid lines by TMT mass spec, finds ~6% of proteins vary and about half of pQTLs overlap eQTLs while the rest act post‑transcriptionally. In yeast a single amino‑acid in YRR1 shifts NQO resistance and partner binding, producing a broad transcriptional program change.

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" chapters (22)

1. 0:00Variation Regulatory Information within and Between Species
2. 0:34Topics 1. Variation in regulatory information among humans - Protein levels 2. Master variators: linking
3. 1:22What is the Difference Between People and Between Species? Coding Sequences vs Regulation
4. 1:51Many Studies Have Demonstrated that Gene Expression Differs Among Individuals and Species Among humans 1 Microarrays: Stranger, Dermitzakis et al. Science (2007), PloS Genetics (2005), Nature
5. 3:03Extensive Differences in TF Binding Between Species
6. 4:14Variability in NFKappaB and Polll loci in Humans
7. 4:56Additional Fundamental Questions?
8. 6:00Histone Marks Representing a Variety of Functional Elements promoter
9. 9:48Chromatin States Learned on 19 Individuals
10. 12:07State Specific Variability of Chromatin Marks
11. 17:04Chromatin Marks are Highly Heritable
12. 18:50Functional Consequences of Chromatin Variation
13. 20:31Map Protein Variation Across Individuals Map Relative Protein levels Across 90 LC Lines Using TMT Labeling
14. 24:09Identify 77 pQTLs with 10%FDR; 273 with 30% FDR
15. 27:30Personal Phenotypes: Variation in Chemical Response
16. 29:02Genome-wide analysis study finds one region linked to NQO response
17. 31:00Allele swap
18. 35:30Comparing Mouse and Human with Mouse ENCODE Data
19. 41:34Conservation of occupancy by GATA factors predicts enhancers active in multiple tissues
20. 42:43Much of the biochemical activity covering a substantial portion of the genome is low level
21. 43:24Higher signals for biochemical activity in more conserved genomic regions
22. 44:09Acknowledgements

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